<DIV>Hi, dock fans, I ran flexiable docking with dock6.1 and have ran into a problem below :</DIV>
<DIV><EM>ERROR: Could not complete growth. </EM></DIV>
<DIV><I> Confirm grid box is large enough to contain <BR></I><I> ligand and try increasing max_orients.<BR></DIV></I>
<DIV> </DIV>
<DIV>I read messages from :<A href="http://blur.compbio.ucsf.edu/pipermail/dock-fans/2006-August/000694.html">http://blur.compbio.ucsf.edu/pipermail/dock-fans/2006-August/000694.html</A> , which said turning off the bump checking to fix the problem , but the problem is that I used "bump_filter no" in my parameter file, the following is the parameters I used . Is there something wrong?</DIV>
<DIV> </DIV>
<DIV>--------------------------------------<BR>DOCK v6.1</DIV>
<DIV>Released December 2006<BR>Copyright UCSF<BR>--------------------------------------</DIV>
<DIV><BR>Molecule Library Input Parameters<BR>------------------------------------------------------------------------------------------<BR>ligand_atom_file dru.mol2<BR>limit_max_ligands no<BR>skip_molecule no<BR>read_mol_solvation no<BR>calculate_rmsd no</DIV>
<DIV>Orient Ligand Parameters<BR>------------------------------------------------------------------------------------------<BR>orient_ligand yes<BR>automated_matching yes<BR>receptor_site_file ../2_site/rec.sph<BR>max_orientations 5000<BR>critical_points no<BR>chemical_matching no<BR>use_ligand_spheres no</DIV>
<DIV>Flexible Ligand Parameters<BR>------------------------------------------------------------------------------------------<BR></DIV>
<DIV>flexible_ligand yes<BR>min_anchor_size 15<BR>pruning_use_clustering yes<BR>pruning_max_orients 100<BR>pruning_clustering_cutoff 100<BR>use_internal_energy yes<BR>internal_energy_att_exp 6<BR>internal_energy_rep_exp 12<BR>internal_energy_dielectric 4.0<BR>use_clash_overlap no</DIV>
<DIV>Bump Filter Parameters<BR>------------------------------------------------------------------------------------------<BR>bump_filter no</DIV>
<DIV>Master Score Parameters<BR>------------------------------------------------------------------------------------------<BR>score_molecules yes</DIV>
<DIV>Contact Score Paramters<BR>------------------------------------------------------------------------------------------<BR>contact_score_primary no<BR>contact_score_secondary no</DIV>
<DIV>Grid Score Parameters<BR>------------------------------------------------------------------------------------------<BR>grid_score_primary yes<BR>grid_score_secondary no<BR>grid_score_rep_rad_scale 1<BR>grid_score_vdw_scale 1<BR>grid_score_es_scale 1<BR>grid_score_grid_prefix ../3_grid/grid</DIV>
<DIV>Dock3.5 Score Parameters<BR>------------------------------------------------------------------------------------------<BR>dock3.5_score_secondary no</DIV>
<DIV>Continuous Energy Score Parameters<BR>------------------------------------------------------------------------------------------<BR>continuous_score_secondary no</DIV>
<DIV>Zou GB/SA Score Parameters<BR>------------------------------------------------------------------------------------------<BR>gbsa_zou_score_secondary no</DIV>
<DIV>Hawkins GB/SA Score Parameters<BR>------------------------------------------------------------------------------------------<BR>gbsa_hawkins_score_secondary no</DIV>
<DIV>Amber Score Parameters<BR>------------------------------------------------------------------------------------------<BR>amber_score_secondary no</DIV>
<DIV>Warning: No secondary scoring function selected.</DIV>
<DIV><BR>Simplex Minimization Parameters<BR>------------------------------------------------------------------------------------------<BR>minimize_ligand yes<BR>minimize_anchor yes<BR>minimize_flexible_growth yes<BR>use_advanced_simplex_parameters no<BR>simplex_max_cycles 1<BR>simplex_score_converge 0.1<BR>simplex_cycle_converge 1.0<BR>simplex_trans_step 1.0<BR>simplex_rot_step 0.1<BR>simplex_tors_step 10.0<BR>simplex_anchor_max_iterations 500<BR>simplex_grow_max_iterations 500<BR>simplex_final_min no<BR>simplex_random_seed 0</DIV>
<DIV>Atom Typing Parameters<BR>------------------------------------------------------------------------------------------<BR>atom_model all<BR>vdw_defn_file ../../parameters/vdw_AMBER_parm99.defn<BR>flex_defn_file ../../parameters/flex.defn<BR>flex_drive_file ../../parameters/flex_drive.tbl</DIV>
<DIV>Molecule Library Output Parameters<BR>------------------------------------------------------------------------------------------<BR>ligand_outfile_prefix druggable<BR>write_orientations no<BR>num_scored_conformers_written 1<BR>rank_ligands no<BR>------------------------------------------------------------------------------------------</DIV>
<DIV>Initializing Library File Routines...<BR>Initializing Orienting Routines...<BR>Initializing Conformer Generator Routines...<BR>Initializing Grid Score Routines...<BR> Reading the energy grid from ../3_grid/grid.nrg</DIV>
<DIV>-----------------------------------<BR>Molecule: AST 4892090</DIV>
<DIV> Elapsed time: 40 seconds</DIV>
<DIV> ERROR: Could not complete growth. <BR> Confirm grid box is large enough to contain <BR> ligand and try increasing max_orients.</DIV>
<DIV><BR>1 Molecules Processed<BR><BR><BR></DIV>